Protein nanobarcodes enable single-step multiplexed fluorescence imaging

de Jong-Bolm, D.; Sadeghi, M.; Bao, G.; Klaehn, G.; Hoff, M.; Mittelmeier, L.; Basmanav, F. B.; Opazo, F.; Noe, F.; Rizzoli, S. O.

doi:10.1101/2022.06.03.494744

bioRxiv · 10.1101/2022.06.03.494744

Protein nanobarcodes enable single-step multiplexed fluorescence imaging

de Jong-Bolm, D.·Sadeghi, M.·Bao, G.·Klaehn, G.·Hoff, M.·Mittelmeier, L.·Basmanav, F. B.·Opazo, F.·Noe, F.·Rizzoli, S. O.

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Abstract

Multiplexed cellular imaging typically relies on the sequential application of detection probes, such as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations.

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de Jong-Bolm, D., Sadeghi, M., Bao, G., Klaehn, G., Hoff, M., Mittelmeier, L., Basmanav, F. B., Opazo, F., Noe, F., Rizzoli, S. O.. 2022-06-04. Protein nanobarcodes enable single-step multiplexed fluorescence imaging. https://doi.org/10.1101/2022.06.03.494744

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