Exploring Xylem Anatomical Adaptations Associated with Crassulacean Acid Metabolism and Hydraulic Capacitance in Clusia Leaves: Lessons for CAM Bioengineering

Abstract

* Background and AimsMany succulent species are characterised by the presence of crassulacean acid metabolism (CAM) and/or elevated bulk hydraulic capacitance (CFT). Both CAM and elevated CFT substantially reduce the rate at which water moves through transpiring leaves. However, little is known about how these physiological adaptations are coordinated with leaf vascular architecture and xylem anatomy. * MethodsThe genus Clusia contains species spanning the entire C3-CAM continuum, and also is known to have > 5-fold interspecific variation in CFT. We used this highly diverse genus to explore how interspecific variation in vein density and xylem vessel dimensions are coordinated with CAM and CFT. * Key ResultsWe found that constitutive CAM phenotypes were associated with lower vein length per leaf area (VLA) and vein termini density (VTD), compared to C3 or facultative CAM species. However, when vein densities were standardised by leaf depth, this value was higher in CAM than C3 species, which is likely an adaptation to overcome apoplastic hydraulic resistance in deep chlorenchyma tissue. In contrast, CFT did not correlate with any xylem anatomical trait measured, suggesting CAM has a greater impact on leaf hydraulic physiology than CFT. * ConclusionsOur findings strongly suggest that CAM photosynthesis is coordinated with leaf vein densities. The link between CAM and vascular anatomy will be important to consider when attempting to bioengineer CAM into C3 crops.