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bioRxiv · 10.1101/2022.05.25.493454

Rapid and precise genome engineering in a naturally short-lived vertebrate

Nath, R. D.·Bedbrook, C. N.·Nagvekar, R.·Deisseroth, K.·Brunet, A.
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Abstract

The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci, to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.

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Nath, R. D., Bedbrook, C. N., Nagvekar, R., Deisseroth, K., Brunet, A.. 2022-05-26. Rapid and precise genome engineering in a naturally short-lived vertebrate. https://doi.org/10.1101/2022.05.25.493454

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