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bioRxiv · 10.1101/2022.05.22.493006

Single-Cell Transcriptome Analyses Reveal the Cell Diversity and Developmental Features of Human Gastric and Metaplastic Mucosa

Tsubosaka, A.·Komura, D.·Katoh, H.·Kakiuchi, M.·Onoyama, T.·Yamamoto, A.·Abe, H.·Seto, Y.·Ushiku, T.·Ishikawa, S.
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Abstract

The stomach is an important digestive organ with a variety of biological functions. However, due to the complexity of its cellular and glandular composition, the precise cellular biology has yet to be elucidated. In this study, we conducted single-cell RNA sequence analysis of the human stomach and constructed a 137,610-cell dataset, the largest cell atlas reported to date. By integrating this single-cell analysis with spatial cellular distribution analysis, we were able to clarify novel aspects of the developmental and tissue homeostatic ecosystems in the human stomach. We identified LEFTY1+ as a potential stem cell marker in both gastric and intestinal metaplastic glands. We also revealed skewed distribution patterns for PDGFRA+BMP4+WNT5A+ fibroblasts that play pivotal roles in, or even precede, the phenotypic changes from gastric to metaplastic mucosa. Our extensive dataset will function as a fundamental resource in investigations of the stomach, including studies on development, aging, and carcinogenesis.

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Tsubosaka, A., Komura, D., Katoh, H., Kakiuchi, M., Onoyama, T., Yamamoto, A., Abe, H., Seto, Y., Ushiku, T., Ishikawa, S.. 2022-05-23. Single-Cell Transcriptome Analyses Reveal the Cell Diversity and Developmental Features of Human Gastric and Metaplastic Mucosa. https://doi.org/10.1101/2022.05.22.493006

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