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bioRxiv · 10.1101/2022.05.22.492888

Alzheimer's disease rewires gene coexpression networks coupling different brain regions

Mitra, S.·B P, K.·C R, S.·Saikumar, N. V.·Philip, P.·Narayanan, M.
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Abstract

Connectome studies have revealed how neurodegenerative diseases like Alzheimers disease (AD) disrupt functional and structural connectivity among brain regions, but the molecular basis of such disruptions is less studied, with most genomic studies focusing on within-brain-region molecular analyses. We performed an inter-brain-region differential correlation (DC) analysis of postmortem human brain RNA-seq data available for four brain regions - frontal pole, superior temporal gyrus, parahippocampal gyrus, and inferior frontal gyrus - from Mount Sinai Brain Bank for hundreds of AD vs. control samples. For any two brain regions, our DC analysis identifies all pairs of genes across these regions whose coexpression/correlation strength in the AD group differs significantly from that in the Control group, after adjusting for cell type compositional effects to better capture cell-intrinsic changes. Such DC gene pairs provided information complementary to known differentially expressed genes in AD, and highlighted extensive rewiring of the network of cross-region coexpression-based couplings among genes. The most vulnerable region in AD, parahippocampal gyrus, showed the most rewiring in its coupling with other brain regions. Decomposing the DC network into bipartite (region-region) gene modules revealed enrichment for synaptic signaling and ion transport pathways in several modules, revealing the dominance of five genes (BSN, CACNA1B, GRIN1, IQSEC2, and SYNGAP1). AD cerebrospinal fluid biomarkers (AD-CSF), neurotransmitters, secretory proteins, ligand and receptors were found to be part of the DC network, suggesting how pathways comprising such signaling molecules could mediate region-region communication. A module enriched for AD GWAS (Genome-wide Association Studies) signals is also enriched for NF-{kappa}{beta} signaling pathway, a key mediator of brain inflammation in AD. Beyond modules, we also identified individual genes that act as hubs of AD dysregulation across regions, such as ZKSCAN1 (Zinc Finger with KRAB And SCAN Domains) - this gene is known to be linked to AD in GWAS studies but via unknown mechanisms, and the specific DC interactions of ZKSCAN1 found in this study can be used to dissect these mechanisms. Thus, our inter-region DC framework provides a valuable new perspective to comprehend AD aetiology.

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Mitra, S., B P, K., C R, S., Saikumar, N. V., Philip, P., Narayanan, M.. 2022-05-24. Alzheimer's disease rewires gene coexpression networks coupling different brain regions. https://doi.org/10.1101/2022.05.22.492888

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