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bioRxiv · 10.1101/2022.05.19.492257

Held out wings RNA binding activity in the cytoplasm during early spermatogenesis

Agapiou, M.·Hopes, T.·Hobor, F.·Bretman, A.·edwards, t. a.·Aspden, J. L.
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Abstract

Held out wings (HOW) is an RNA-binding protein essential for spermatogenesis in Drosophila melanogaster. HOW is a signal transduction and activation of RNA (STAR) protein, regulating post-transcriptional gene expression. The characteristics of RNA-binding by the conserved short cytoplasmic isoform, HOW(S), are unknown. In vivo RIP-seq identified 121 novel transcripts bound by HOW(S) in germ stem cells and spermatogonia, many with signal transduction functions. (A/G/U)CUAAC motifs were enriched in 3-UTRs and GCG(A/U)G in 5-UTRs. HOW binds with high-affinity to sites containing CUAAC motifs from lola and hipk mRNAs. This study provides new insight into STAR protein-RNA interactions and functions in spermatogenesis.

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Agapiou, M., Hopes, T., Hobor, F., Bretman, A., edwards, t. a., Aspden, J. L.. 2022-05-19. Held out wings RNA binding activity in the cytoplasm during early spermatogenesis. https://doi.org/10.1101/2022.05.19.492257

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