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bioRxiv · 10.1101/2022.05.18.492426

High-fidelity optical diffraction tomography of live organisms using non-toxic tunable refractive index media

Lee, D.·Lee, M.·KWAK, H.·Kim, Y.·Shim, J.·Jung, J. H.·Park, W.-S.·Park, J.-H.·Lee, S.·Park, Y.
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Abstract

Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization of live biological samples without producing toxic effects. We validate our method using various biological organisms, including C. albicans and C. elegans. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=139 SRC="FIGDIR/small/492426v1_ufig1.gif" ALT="Figure 1"> View larger version (45K): org.highwire.dtl.DTLVardef@a6b4d4org.highwire.dtl.DTLVardef@a9ec7borg.highwire.dtl.DTLVardef@1e4a795org.highwire.dtl.DTLVardef@b663aa_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Lee, D., Lee, M., KWAK, H., Kim, Y., Shim, J., Jung, J. H., Park, W.-S., Park, J.-H., Lee, S., Park, Y.. 2022-05-18. High-fidelity optical diffraction tomography of live organisms using non-toxic tunable refractive index media. https://doi.org/10.1101/2022.05.18.492426

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