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bioRxiv · 10.1101/2022.05.17.492343

Comparison of simple RNA extraction methods for molecular diagnosis of hepatitis C virus in plasma

Hongjaisee, S.·Jabjainai, Y.·Sakset, S.·Preechasuth, K.·Ngo-Giang-Huong, N.·Khamduang, W.
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Abstract

We evaluated the efficacy of four simple RNA extraction methods for the detection of hepatitis C virus (HCV) in plasma samples: silica-membrane-based, magnetic beads-based, boiling with Diethyl Pyrocarbonate (DEPC)-treated distilled water or a commercial lysis buffer. HCV RNA was detected using both real time reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop mediated isothermal amplification (RT-LAMP). The magnetic beads-based extraction can be used as an alternative RNA extraction method for on-site HCV detection. Boiling with DEPC-treated distilled water was not appropriate for low HCV load samples and boiling with a lysis buffer was not recommended.

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Hongjaisee, S., Jabjainai, Y., Sakset, S., Preechasuth, K., Ngo-Giang-Huong, N., Khamduang, W.. 2022-05-17. Comparison of simple RNA extraction methods for molecular diagnosis of hepatitis C virus in plasma. https://doi.org/10.1101/2022.05.17.492343

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