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bioRxiv · 10.1101/2022.05.10.491248

Reconstruction of Full-length scFv Libraries with the Extended Range Targeted Sequencing Method

Wei, C.·Criner, S.·Sridhar, B.·Shultzaberger, R.·Looney, T.·Fabani, M. M.·Glezer, E. N.
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Abstract

Single chain fragment variable (scFv) phage display libraries of randomly paired VH-VL antibody domains are a powerful and widely adopted tool for the discovery of antibodies of a desired specificity. Characterization of full length VH-VL constructs using synthetic long read assemblies of short read next-generation sequencing data has emerged as a powerful approach to identify antibody candidates with greater speed and sensitivity than classical screening methods. Here we introduce a new version of the synthetic long read approach, which we denote the Extended Range Targeted Sequencing method. We apply the method to demonstrate accurate and high throughput analysis of full-length VH-VL constructs from a commercial scFv combinatorial display library.

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Wei, C., Criner, S., Sridhar, B., Shultzaberger, R., Looney, T., Fabani, M. M., Glezer, E. N.. 2022-05-10. Reconstruction of Full-length scFv Libraries with the Extended Range Targeted Sequencing Method. https://doi.org/10.1101/2022.05.10.491248

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