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bioRxiv · 10.1101/2022.05.03.490360

Genome Mining and Biochemical Characterization of a Bifunctional Type I Diterpene Synthase from a Marine-Derived Fungus

Zhang, P.·Wu, G.·Heard, S. C.·Niu, C.·Zhang, Y.·Winter, J. M.
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Abstract

We report the identification of the tnd biosynthetic cluster from the marine-derived fungal strain Aspergillus flavipes CNL-338 and in vivo characterization of a cryptic type I diterpene synthase. Heterologous expression of the bifunctional terpene synthase TndC in Saccharomyces cerevisiae led to the discovery of a new diterpene backbone, talarodiene, harboring a benzo[a]cyclopenta[d]cyclooctane tricyclic fused ring system. The cyclization mechanism that converts geranylgeranyl diphosphate to the tricyclic hydrocarbon skeleton was investigated using 13C-labeling studies and stable isotope tracer experiments showed the biotransformation of talarodiene into talaronoid C.

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Zhang, P., Wu, G., Heard, S. C., Niu, C., Zhang, Y., Winter, J. M.. 2022-05-04. Genome Mining and Biochemical Characterization of a Bifunctional Type I Diterpene Synthase from a Marine-Derived Fungus. https://doi.org/10.1101/2022.05.03.490360

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