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bioRxiv · 10.1101/2022.04.28.489834

Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domains

Zhang, Q.·Tiwari, S. K.·Wang, S.·Wang, L.·Li, W.·Zhang, L.·Rawlings, S. A.·Cheng, Y.·Jokerst, J. V.·Rana, T. M.
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Abstract

To address the need for multivalent vaccines against Coronaviridae that can be rapidly developed and manufactured, we compared antibody responses against SARS-CoV, SARS-CoV-2, and several variants of concern in mice immunized with mRNA-lipid nanoparticle vaccines encoding homodimers or heterodimers of SARS-CoV/SARS-CoV-2 receptor-binding domains. All vaccine constructs induced robust anti-viral antibody responses, and the heterodimeric vaccine elicited an IgG response capable of cross-neutralizing SARS-CoV, SARS-CoV-2 Wuhan-Hu-1, B.1.351 (beta), and B.1.617.2 (delta) variants.

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Zhang, Q., Tiwari, S. K., Wang, S., Wang, L., Li, W., Zhang, L., Rawlings, S. A., Cheng, Y., Jokerst, J. V., Rana, T. M.. 2022-04-28. Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domains. https://doi.org/10.1101/2022.04.28.489834

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