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bioRxiv · 10.1101/2022.04.26.489629

Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments

Liang, G.·Yin, H.·Ding, F.
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Abstract

Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, to set up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cell moving out of limited field of view), especially in a long time recording. To overcome these issues, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example system, we have demonstrated that our boundary-free patterned surface solves the cell-losing problem without disturbing their cellular phenotype. Statistically, the presented system increases the effective-throughput of time-lapse microscopy experiments by order of magnitude.

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Liang, G., Yin, H., Ding, F.. 2022-04-28. Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments. https://doi.org/10.1101/2022.04.26.489629

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