Exceptional yield vesicle packaged recombinant protein production from E. coli.

Eastwood, T. A.; Baker, K.; Streather, B. R.; Allen, N.; Wang, L.; Botchway, S. W.; Brown, I. R.; Hiscock, J. R.; Lennon, C.; Mulvihill, D. P.

doi:10.1101/2022.04.22.489142

bioRxiv · 10.1101/2022.04.22.489142

Exceptional yield vesicle packaged recombinant protein production from E. coli.

Eastwood, T. A.·Baker, K.·Streather, B. R.·Allen, N.·Wang, L.·Botchway, S. W.·Brown, I. R.·Hiscock, J. R.·Lennon, C.·Mulvihill, D. P.

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Abstract

We describe a novel system that exports diverse recombinant proteins in extracellular vesicles from E. coli. The vesicles not only compartmentalise toxic, insoluble and disulphide bond containing proteins in a soluble and functional form (e.g. DNaseI, nanobodies and IgG-fusions), but the continued release of the inducible vesicle packaged proteins into the media supports continuous isolation of protein from active culture within a micro-environment allowing stable long-term storage. This technology results in unprecedented yields of vesicle packaged functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.

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Eastwood, T. A., Baker, K., Streather, B. R., Allen, N., Wang, L., Botchway, S. W., Brown, I. R., Hiscock, J. R., Lennon, C., Mulvihill, D. P.. 2022-04-22. Exceptional yield vesicle packaged recombinant protein production from E. coli.. https://doi.org/10.1101/2022.04.22.489142

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