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bioRxiv · 10.1101/2022.04.21.489051

A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice.

Klioutchnikov, A.·Wallace, D. J.·Sawinski, J.·Voit, K.-M.·Groemping, Y.·Kerr, J. N.
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Abstract

Recent advances in head-mounted microscopes have enabled imaging of neuronal activity using genetic-tools in freely moving mice but these microscopes are restricted to recording in minimally lit arenas and imaging upper cortical layers. Here we built a 2 gram, three-photon excitation-based microscope, containing a z-drive that enabled access to all cortical layers while mice freely behaved in a fully lit environment. We show that neuronal population activity in cortical layer-4 and layer-6 was differentially modulated by lit and dark conditions during free exploration.

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Klioutchnikov, A., Wallace, D. J., Sawinski, J., Voit, K.-M., Groemping, Y., Kerr, J. N.. 2022-04-22. A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice.. https://doi.org/10.1101/2022.04.21.489051

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