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bioRxiv · 10.1101/2022.04.21.488890

Ubiquitination occurs in the mitochondrial matrix byeclipsed targeted components of the ubiquitin machinery

Zhang, Y.·Karmon, O.·Das, K.·Mark, M.·Lehming, N.·Pines, O.
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Abstract

Ubiquitination is a critical type of post translational modification in eukaryotic cells. It is involved in regulating nearly all cellular processes in the cytosol and nucleus. Mitochondria, known as the metabolism heart of the cell, are organelles that evolved from bacteria. Using the subcellular compartment-dependent -complementation, we detect multiple components of ubiquitination machinery as being eclipsed distributed to yeast mitochondria. Subsequently, the results with respect to MTS (mitochondrial targeting sequence) targeted HA-tagged ubiquitin demonstrate that certain ubiquitination events specifically occur in yeast mitochondria and are independent of proteasome activity in the cytosol/nucleus. Importantly, we show that the E2 Rad6 affects the pattern of protein ubiquitination in mitochondria and provides an in vivo assay for its activity in the matrix of the organelle. This study shows that ubiquitination occurs in the mitochondrial matrix by eclipsed targeted components of the ubiquitin machinery, providing a new perspective of mitochondrial and ubiquitination research.

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Zhang, Y., Karmon, O., Das, K., Mark, M., Lehming, N., Pines, O.. 2022-04-21. Ubiquitination occurs in the mitochondrial matrix byeclipsed targeted components of the ubiquitin machinery. https://doi.org/10.1101/2022.04.21.488890

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