Canonical Strigolactones Are Not the Tillering-Inhibitory Hormone but Rhizospheric Signals in Rice

Ito, S.; Braguy, J.; Wang, J. Y.; Yoda, A.; Fiorilli, V.; Takahashi, I.; Jamil, M.; Felemban, A.; Miyazaki, S.; Mazzarella, T.; Shinozawa, A.; Balakrishna, A.; Berqdar, L.; Rajan, C.; Ali, S.; Haider, I.; Sasaki, Y.; Yajima, S.; Akiyama, K.; Lanfranco, L.; Zurbriggen, M. D.; Nomura, T.; Asami, T.; Al-Babili, S.

doi:10.1101/2022.04.05.487102

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bioRxiv · 10.1101/2022.04.05.487102

Canonical Strigolactones Are Not the Tillering-Inhibitory Hormone but Rhizospheric Signals in Rice

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Abstract

The plant hormones strigolactones (SLs) regulate shoot branching and mediate the communication with symbiotic mycorrhizal fungi, but also with noxious root parasitic weeds, such as Striga spp. SLs derive from carlactone (CL) and are divided structurally into canonical and non-canonical SLs. However, the questions about particular biological functions of the two groups and the identification of the SL that inhibits shoot branching are still unanswered, hampering targeted modification of SL pattern towards improving plant architecture and resistance against Striga. Here, we reported that 4-deoxyorobanchol (4DO) and orobanchol, the two canonical SLs in rice, do not have major role in determining rice shoot architecture. CRISPR/Cas9 mediated Osmax1-900 mutants, lacking these two SLs, do not show the high tillering and dwarf phenotype typical for SL-deficient plants. However, the absence of 4DO and orobanchol in root exudates significantly decreased their capability in inducing Striga seed germination, while caused only a delay in root colonization by mycorrhizal fungi. To confirm the genetic evidence, we used the SL-biosynthesis inhibitor TIS108. Our results showed that TIS108 is a MAX1-specific inhibitor that lowers 4DO and orobanchol synthesis, conferring a resistance to Striga without a severe impact on rice architecture. Hence, our work uncovers the specific function of canonical SLs as rhizospheric signals and paves the way for establishing chemical and genetic based approaches for combating the root parasitic weeds, by targeted depletion of their release.

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Ito, S., Braguy, J., Wang, J. Y., Yoda, A., Fiorilli, V., Takahashi, I., Jamil, M., Felemban, A., Miyazaki, S., Mazzarella, T., Shinozawa, A., Balakrishna, A., Berqdar, L., Rajan, C., Ali, S., Haider, I., Sasaki, Y., Yajima, S., Akiyama, K., Lanfranco, L., Zurbriggen, M. D., Nomura, T., Asami, T., Al-Babili, S.. 2022-04-07. Canonical Strigolactones Are Not the Tillering-Inhibitory Hormone but Rhizospheric Signals in Rice. https://doi.org/10.1101/2022.04.05.487102

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