Kidins220 regulates the development of B cells bearing the {lambda} light chain

Abstract

The ratio between Ig{kappa} and Ig{lambda} light chain (LC)-expressing B cells varies considerably between species. We recently identified Kinase D-interacting substrate of 220 kDa (Kidins220) as an interaction partner of the BCR. In vivo ablation of Kidins220 in B cells resulted in a marked reduction of {lambda}LC-expressing B cells. Kidins220 knockout B cells fail to open and recombine the genes of the{lambda} LC locus, even in genetic scenarios where the{kappa} LC genes cannot be rearranged or where the {kappa}LC confers autoreactivity.{kappa} LC gene recombination and expression in Kidins220-deficient B cells is normal. Kidins220 regulates the development of {lambda}LC B cells by enhancing the survival of developing B cells and thereby extending the time-window in which the{lambda} LC locus opens and the genes are rearranged and transcribed. Further, our data suggest that Kidins220 guarantees optimal pre-BCR and BCR signaling to induce{lambda} LC locus opening and gene recombination during B cell development and receptor editing. One Sentence SummaryWe demonstrate that the scaffold protein Kidins220 regulates the development of {lambda}LC B cells by supporting B cell precursor survival and optimizing pre-BCR and BCR signaling to open, recombine, and transcribe the genes of the{lambda} LC locus.