Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping

Liu, Y.; Distasio, M.; Su, G.; Asashima, H.; Enninful, A.; Qin, X.; Deng, Y.; Bordignon, P.; Cassano, M.; Tomayko, M.; Xu, M. L.; Halene, S.; Craft, J. E.; Hafler, D. A.; Fan, R.

doi:10.1101/2022.04.01.486788

bioRxiv · 10.1101/2022.04.01.486788

Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping

Liu, Y.·Distasio, M.·Su, G.·Asashima, H.·Enninful, A.·Qin, X.·Deng, Y.·Bordignon, P.·Cassano, M.·Tomayko, M.·Xu, M. L.·Halene, S.·Craft, J. E.·Hafler, D. A.·Fan, R.

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Abstract

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

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Liu, Y., Distasio, M., Su, G., Asashima, H., Enninful, A., Qin, X., Deng, Y., Bordignon, P., Cassano, M., Tomayko, M., Xu, M. L., Halene, S., Craft, J. E., Hafler, D. A., Fan, R.. 2022-04-02. Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping. https://doi.org/10.1101/2022.04.01.486788

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