GTAGMe-seq: joint profiling of genetic variants, DNA methylation, GpC methyltransferase footprints, and 3D genome in the same DNA molecules

Fu, H.; Zheng, H.; Muglia, L. J.; Wang, L.; Liu, Y.

doi:10.1101/2022.03.29.486102

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bioRxiv · 10.1101/2022.03.29.486102

GTAGMe-seq: joint profiling of genetic variants, DNA methylation, GpC methyltransferase footprints, and 3D genome in the same DNA molecules

Fu, H.·Zheng, H.·Muglia, L. J.·Wang, L.·Liu, Y.

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Abstract

Cis-regulatory elements coordinate the regulation of their targeted genes expression. However, the joint measurement of cis-regulatory elements activities and their interactions in spatial proximity is limited by the current sequencing approaches. We describe a method, NOMe-HiC, which simultaneously captures single nucleotide polymorphisms, DNA methylation, chromatin accessibility (GpC methyltransferase footprints), and chromosome conformation changes from the same DNA molecule, together with the transcriptome, in a single assay. NOMe-HiC shows high concordance with state-of-the-art mono-omic assays across different molecular measurements and reveals coordinated chromatin accessibility at distal genomic segments in spatial proximity and novel types of long-range allele-specific chromatin accessibility.

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Fu, H., Zheng, H., Muglia, L. J., Wang, L., Liu, Y.. 2022-03-30. GTAGMe-seq: joint profiling of genetic variants, DNA methylation, GpC methyltransferase footprints, and 3D genome in the same DNA molecules. https://doi.org/10.1101/2022.03.29.486102

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