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bioRxiv · 10.1101/2022.03.28.486031

A widely applicable and cost-effective method for general and specific RNA-protein complex isolation

Balzarini, S.·Van Ende, R.·Voet, A. R.·Geuten, K.
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Abstract

Despite important methodological advances made in the past few years, a widely applicable, cost-effective and easily scalable procedure that can be routinely used to isolate ribonucleoprotein complexes (RNPs) remains elusive. We describe a versatile method that connects aspects of existing methods in a workflow optimized to reach the above goals and called it "Silica-based Acidic Phase Separation (SAPS)-capture". To validate the method, the 18S rRNP of S. cerevisiae was captured. To illustrate its applicability, we isolated a repertoire of RNPs in A. thaliana. This procedure can provide the community with a powerful tool to advance the study of ribonomes and RNPs in any organism or tissue type.

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Balzarini, S., Van Ende, R., Voet, A. R., Geuten, K.. 2022-03-28. A widely applicable and cost-effective method for general and specific RNA-protein complex isolation. https://doi.org/10.1101/2022.03.28.486031

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