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bioRxiv · 10.1101/2022.03.26.485930

Complete sequencing of the mouse pseudoautosomal region, the most rapidly evolving 'chromosome'

Kasahara, T.·Mekada, K.·Abe, K.·Ashworth, A.·Kato, T.
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Abstract

The pseudoautosomal region (PAR) of mammalian sex chromosomes is a small region of sequence identity that allows pairing, crossover, recombination, and proper chromosome segregation during male meiosis. The structure of the mouse PAR is largely unknown. Here, we developed a new assembly method to robustly resolve repetitive sequences and employed highly accurate long-read sequencing data to reveal the entire PAR sequence. The PAR of the widely-used inbred strain C57BL/6J is [~]700 kb, comprising 10 protein- coding genes in a mass of complex repetitive sequences. A large segmental duplication exhibiting copy-number polymorphisms even among C57BL/6J littermates is present. High GC-content exons and short introns are common properties of PAR genes and are the consequence of maintaining gene function, while PAR is rapidly evolving. Elucidating the mouse PAR sequence completes the mouse euchromatic genome sequencing and enables the exploration of the function and evolution of the PAR using modern molecular genetic approaches.

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Kasahara, T., Mekada, K., Abe, K., Ashworth, A., Kato, T.. 2022-03-27. Complete sequencing of the mouse pseudoautosomal region, the most rapidly evolving 'chromosome'. https://doi.org/10.1101/2022.03.26.485930

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