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bioRxiv · 10.1101/2022.03.25.485787

circRAB3IP modulates cell proliferation by reorganizing gene expression and mRNA processing in a paracrine manner

Josipovic, N.·Ebbessen, K. K.·Zirkel, A.·Mackay-Danieli, A.·Dieterich, C.·Kurian, L.·Hansen, T. B.·Papantonis, A.
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Abstract

Circular RNAs are endogenous long-lived and abundant non-coding species. Despite their prevalence, only few circRNAs have been dissected mechanistically to date. Here, we catalogued nascent RNA-enriched circRNAs from primary human cells and functionally assign a role to circRAB3IP in sustaining cellular homeostasis. We combined "omics" and functional experiments to show how circRAB3IP depletion deregulates hundreds of genes, suppresses cell cycle progression, and induces senescence-associated gene expression changes. Conversely, excess circRAB3IP delivered to endothelial cells via extracellular vesicles suffices for accelerating their division. We attribute these effects to the interplay between circRAB3IP and the general splicing factor SF3B1, which impacts transcript usage of cell cycle-related genes. Together, our findings link the maintenance of cell homeostasis to the tightly regulated titers of a single circRNA.

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Josipovic, N., Ebbessen, K. K., Zirkel, A., Mackay-Danieli, A., Dieterich, C., Kurian, L., Hansen, T. B., Papantonis, A.. 2022-03-27. circRAB3IP modulates cell proliferation by reorganizing gene expression and mRNA processing in a paracrine manner. https://doi.org/10.1101/2022.03.25.485787

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