A cellular and molecular analysis of SoxB-driven neurogenesis in a cnidarian

Frank, U.; Chrysostomou, E.; Gornik, S.; Salinas-Saavedra, M.; Gahan, J.; Flici, H.; McMahon, E.; Thompson, K.; Hanley, S.; Kincoyne, M.; Schnitzler, C.; Gonzalez, P.; Baxevanis, A.

doi:10.1101/2022.03.22.485278

bioRxiv · 10.1101/2022.03.22.485278

A cellular and molecular analysis of SoxB-driven neurogenesis in a cnidarian

Frank, U.·Chrysostomou, E.·Gornik, S.·Salinas-Saavedra, M.·Gahan, J.·Flici, H.·McMahon, E.·Thompson, K.·Hanley, S.·Kincoyne, M.·Schnitzler, C.·Gonzalez, P.·Baxevanis, A.

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Abstract

Neurogenesis is the generation of neurons from stem cells, a process that is regulated by SoxB transcription factors (TFs) in many animals. Although the roles of these TFs are well understood in bilaterians, how their neural function evolved is unclear. Here, we use Hydractinia symbiolongicarpus, a member of the early-branching phylum Cnidaria, to provide insight into this question. Using a combination of mRNA in situ hybridization, transgenesis, gene knockdown, transcriptomics, and in-vivo imaging, we provide a comprehensive molecular and cellular analysis of neurogenesis during embryogenesis, homeostasis, and regeneration in this animal. We show that SoxB genes act sequentially. Stem cells expressing Piwi1 and Soxb1, which have a broad developmental potential, become neural progenitors that express Soxb2 before differentiating into mature neural cells. Knockdown of SoxB genes resulted in complex defects in embryonic neurogenesis. Hydractinia neural cells differentiate while migrating from the aboral to the oral end of the animal, but it is unclear whether migration per se or exposure to different microenvironments is the main driver of their fate determination. Our data constitute a rich resource for studies aiming at addressing this question, which is at the heart of understanding neurological disorders in all animals, including humans.

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Frank, U., Chrysostomou, E., Gornik, S., Salinas-Saavedra, M., Gahan, J., Flici, H., McMahon, E., Thompson, K., Hanley, S., Kincoyne, M., Schnitzler, C., Gonzalez, P., Baxevanis, A.. 2022-03-24. A cellular and molecular analysis of SoxB-driven neurogenesis in a cnidarian. https://doi.org/10.1101/2022.03.22.485278

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