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bioRxiv · 10.1101/2022.03.21.485119

Deep topographic proteomics of a human brain tumour

Davis, S.·Scott, C.·Oetjen, J.·Charles, P. D.·Kessler, B. M.·Ansorge, O.·Fischer, R.
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Abstract

The spatial organisation of cellular protein expression profiles within tissue determines cellular function and is key to understanding disease pathology. To define molecular phenotypes in the spatial context of tissue, there is a need for unbiased, quantitative technology capable of mapping proteomes within tissue structures. Here, we present a workflow for spatially-resolved, quantitative proteomics of tissue that generates maps of protein abundance across tissue slices derived from a human atypical teratoid-rhabdoid tumour (AT/RT). We employ spatially-aware algorithms that do not require prior knowledge of the fine tissue structure to detect proteins and pathways with spatial abundance patterns. We identified PYGL, ASPH and CD45 as spatial markers for tumour boundary and reveal immune response-driven, spatially-organised protein networks of the extracellular tumour matrix. Overall, this work informs on methods for spatially resolved deep proteo-phenotyping of tissue heterogeneity, to push the boundaries of understanding tissue biology and pathology at the molecular level.

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Davis, S., Scott, C., Oetjen, J., Charles, P. D., Kessler, B. M., Ansorge, O., Fischer, R.. 2022-03-21. Deep topographic proteomics of a human brain tumour. https://doi.org/10.1101/2022.03.21.485119

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