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bioRxiv · 10.1101/2022.03.10.483652

Open modification searching of SARS-CoV-2-human protein interaction data reveals novel viral modification sites

Adams, C.·Boonen, K.·Laukens, K.·Bittremieux, W.
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Abstract

The outbreak of the SARS-CoV-2 coronavirus, the causative agent of the COVID-19 disease, has led to an ongoing global pandemic since 2019. Mass spectrometry can be used to understand the molecular mechanisms of viral infection by SARS-CoV-2, for example, by determining virus-host protein-protein interactions (PPIs) through which SARS-CoV-2 hijacks its human hosts during infection, and to study the role of post-translational modifications (PTMs). We have reanalyzed public affinity purification mass spectrometry data using open modification searching to investigate the presence of PTMs in the context of the SARS-CoV-2 virus-host PPI network. Based on an over two-fold increase in identified spectra, our detected protein interactions show a high overlap with independent mass spectrometry-based SARS-CoV-2 studies and virus-host interactions for alternative viruses, as well as previously unknown protein interactions. Additionally, we identified several novel modification sites on SARS-CoV-2 proteins that we investigated in relation to their interactions with host proteins. A detailed analysis of relevant modifications, including phosphorylation, ubiquitination, and S-nitrosylation, provides important hypotheses about the functional role of these modifications during viral infection by SARS-CoV-2.

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Adams, C., Boonen, K., Laukens, K., Bittremieux, W.. 2022-03-10. Open modification searching of SARS-CoV-2-human protein interaction data reveals novel viral modification sites. https://doi.org/10.1101/2022.03.10.483652

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