bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2022.03.07.482483

Whole-genome sequencing of Bacillus thuringiensis strain SY49.1 reveals the detection of novel candidate pesticidal and bioactive compounds isolated from Turkey

Yilmaz, S.·Idris, A. B.·Ayvaz, A.·Temizgul, R.·Hassan, M. A.
View original source ↗Export metadata ↓

Abstract

BackgroundBacillus thuringiensis SY49.1 (Bt SY49.1) strain is a new strain isolated from a soil sample in Adana city which is nestled in the most fertile agricultural area in Turkey. This strain has insecticidal activity against insect pests from different orders. Also, it is characterized by its significant activity against plant fungal infections and as plant growth promotion. AimsTo report the whole genome sequencing (WGS) and annotation of the Bt SY49.1 strain. Materials and MethodsThe Bt SY49.1 strain was isolated from the soil sample in Adana city by using a sodium acetate enriched medium. Bacterial DNA was extracted then sequenced using Illumina HiSeq technology. After data filtering and quality control, de novo assembly and genomic annotation were applied. Evolutionary and comparative genomic analysis and variant calling were performed using various in silico tools. ResultsThe WGS of strain Bt SY49.1 is 6. 32 Mbp long with a GC content of 34.68%. It reveals large numbers of single nucleotide polymorphisms (SNPs) and InDels. The assembled genome contains 6,562 protein-encoding genes. In addition, it encodes various gene inventories for the biosynthesis of bioactive compounds such as insecticidal delta endotoxins, growth stimulatory deaminase and phosphatases, fungicidal thuricin, bacillibactin, petrobactin, fengycin / plipastin, and zwittermycin A. ConclusionThe strain Bt SY49.1 could have several potential utilities as a source of antibiotics compounds, plant growth promoting metabolites, and biocontrol agents for fungal phytopathogens, and insects. We expect that the draft genome of the Bt SY49.1 strain may provide a model for proper understanding and studying of antimicrobial compound mining, genetic diversity among the B. cereus group, and pathogenicity against insect pests and plant diseases, and growth-promoting activity.

Explore related subjects

genomicsPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Yilmaz, S., Idris, A. B., Ayvaz, A., Temizgul, R., Hassan, M. A.. 2022-03-07. Whole-genome sequencing of Bacillus thuringiensis strain SY49.1 reveals the detection of novel candidate pesticidal and bioactive compounds isolated from Turkey. https://doi.org/10.1101/2022.03.07.482483

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

A Y-specific translocated gsdf copy is a candidate master sex-determining gene in common dab (Limanda limanda)

Sex-determining systems in flatfishes are highly diverse, and even closely related species can differ in both sex-determining loci and heterogametic systems. In common dab (Limanda limanda), the genetic basis of sex determination has not yet been established. Here, we investigated sex determination using pooled whole-genome sequencing combined with long-read genome sequencing of females and males from Iceland and Norway. Genome-wide allele-frequency analyses identified sex-associated regions consistent with male heterogamety (XX/XY), with the strongest signal on chromosome 16. Comparison of the existing reference assembly, which represents an X haplotype at this locus, with a haplotype-resolved male PacBio HiFi assembly revealed a [~]21.5 kb Y-specific insertion on chromosome 16. The insertion showed complete coverage in male pools but little or no coverage in female pools from either population. It contained a translocated genomic segment including a copy of gonadal soma-derived factor (gsdf) and a predicted gene of unknown function. Comparative genomic analyses were consistent with this segment originating from an autosomal gsdf-containing region on chromosome 1. Given the recurrent recruitment of gsdf as a sex-determining factor in teleosts, we designate the Y-linked copy gsdfY and identify it as the strongest candidate for the master sex-determining gene in common dab. Additional broad sex-associated differentiation on chromosome 19 was restricted to the Icelandic samples and may reflect population-specific expansion of the sex-linked region, although its structural basis remains unresolved. Together, these findings provide the first genomic characterization of sex determination in common dab and demonstrate how gene duplication and translocation can contribute to the evolution of novel sex-determining systems in flatfishes.

genomics↗
bioRxiv2026

A population-scale isoform atlas enables isoform-based trait association and regulatory analysis in lettuce

Genomic variation shapes phenotypic diversity, yet how genetic variation contributes to transcript isoform diversity remains poorly understood at the population scale. Here, we integrated transcriptomic data from 240 lettuce accessions with a lettuce pangenome to construct a comprehensive pan isoform atlas comprising 531,657 isoforms from 49,729 transcribed loci. Isoform presence absence variation (iPAV) captured extensive transcript diversification during domestication and horticultural differentiation, providing additional resolution beyond locus-level analyses, particularly within loci shared across the population. To facilitate the systematic analysis of isoform trait associations, we developed ISONET-GWAS, a computational framework that integrates isoform-based association mapping, functional annotation, and network-based candidate prioritization. ISONET-GWAS recovered the known chlorophyll regulator LsGLK, identified 455 metabolite isoform associations across 77 metabolites, and prioritized candidate regulators of chlorophyll metabolism supported by functional validation. Genome-wide splicing quantitative trait locus (sQTL) mapping identified 10,498 sQTL associations, revealing widespread distal genetic associations with alternative splicing and candidate trans-sQTL hotspots. Notably, we identified a trans-sQTL hotspot overlapping LsPPR, which encodes a pentatricopeptide repeat RNA-binding protein. Perturbation of LsPPR resulted in widespread changes in alternative splicing of floweing-related genes, accompanied by delayed bolting and flowering and altered splicing of flowering-related genes. Together, our study establishes a population-scale pan-isoform resource and a broadly applicable analytical strategy for isoform-level trait association, candidate prioritization, and complementary analysis of the genetic regulation of alternative splicing in crops.

genomics↗
bioRxiv2026

Lifestyle-mediated shifts in the gut microbiome and host genetics modulate gene expression in intestinal organoids

Industrialization-associated shifts in the gut microbiome have been linked to disease, but the mechanisms by which microbial communities influence host gene regulation remain poorly understood. We developed a live microbiome-colonic organoid (colonoid) co-culture system to investigate genotype-dependent host responses to gut microbial community variation. Colonoid 2D monolayers from healthy donors were co-cultured with live gut microbial communities from individuals living urban or rural lifestyles, followed by RNA-sequencing. Urban and rural microbiomes elicited largely shared transcriptional responses, however they induced distinct host genetic effects on gene expression. We identified 511 host genes with microbiome-dependent allele-specific expression (ASE) which were involved in epithelial architecture, cell-matrix interactions and structural integrity. Notably, 149 host genes had distinct allelic effects between Urban and Rural microbiomes (urban-rural condition specific ASE or cASE), revealing genotype-dependent host responses to lifestyle-associated microbiomes. Urban-rural cASE was associated with microbial community composition, predominantly involving Prevotella and Bacteroides species. Targeted Prevotella copri spike-in recapitulated Prevotella-associated allelic effects at specific loci, including one in the IBD-associated autophagy gene, ATG4B. Together, these findings establish an experimental framework to investigate genotype-by-microbiome interactions, and demonstrate that lifestyle-mediated shifts in the microbiome modify host cis-regulatory genetic effects on gene expression.

genomics↗