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bioRxiv · 10.1101/2022.03.02.482603

Organoid-based human stomach micro-physiological system to recapitulate the dynamic mucosal defense mechanism

Jeong, H.-J.·Park, J.-H.·Kang, J. H.·Kong, S.-H.·Park, T.-E.
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Abstract

Several stomach diseases are attributed to the dysregulation of physiological function of gastric mucosal barrier by pathogens. Gastric organoids are a promising tool to develop treatment strategies for gastric infections. However, their functional features of in vivo gastric mucosal barrier and host-microbe interactions are limited due to the lack of physiological stimuli. Herein, we describe the first human stomach micro-physiological system (hsMPS) with physiologically relevant gastric mucosal defense system based on the combination of organoid and MPS technology. A fluid flow enhanced epithelial-mesenchymal interaction in the hsMPS enables functional maturation of gastric epithelial cells, which allows for the recreation of mesh-like mucus layer containing high level of mucus protective peptides and well-developed epithelial junctional complexes. Furthermore, gastroprotection mechanisms against Helicobacter pylori are successfully demonstrated in our system. Therefore, hsMPS represents a new in vitro tool for research where gastric mucosal defense mechanism is pivotal for developing therapeutic strategies.

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Jeong, H.-J., Park, J.-H., Kang, J. H., Kong, S.-H., Park, T.-E.. 2022-03-03. Organoid-based human stomach micro-physiological system to recapitulate the dynamic mucosal defense mechanism. https://doi.org/10.1101/2022.03.02.482603

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