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bioRxiv · 10.1101/2022.02.28.482286

Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly-dT termination signals

Girbig, M.·Xie, J.·Groetsch, H.·Libri, D.·Porrua, O.·Mueller, C. W.
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Abstract

RNA polymerase (Pol) III is specialized to transcribe short, abundant RNAs, for which it terminates transcription on poly-thymine (dT) stretches on the non-template (NT) strand. When Pol III reaches the termination signal, it pauses and forms the pre-termination complex (PTC). Here, we report cryo-EM structures of the yeast Pol III PTC and complementary functional states at 2.7-3.9 [A] resolution. Pol III recognizes the poly-dT termination signal with subunit C128 that forms a hydrogen-bond network with the NT-strand and, thereby, induces pausing. Mutating key interacting residues interferes with transcription termination in vitro, impairs yeast growth, and causes global termination defects in vivo confirming our structural results. Additional cryo-EM analysis reveals that C53-C37, a Pol III subcomplex and key termination factor, participates indirectly in Pol III termination. We propose a mechanistic model of Pol III transcription termination and rationalize why Pol III, unlike Pol I and Pol II, terminates on poly-dT signals.

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Girbig, M., Xie, J., Groetsch, H., Libri, D., Porrua, O., Mueller, C. W.. 2022-02-28. Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly-dT termination signals. https://doi.org/10.1101/2022.02.28.482286

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