Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing

Chen, C. X.- Q.; You, Z.; Abdian, N.; Sirois, J.; Shlaifer, I.; Tabatabaei, M.; Boivin, M.-N.; Gaborieau, L.; Karamchandani, J.; Beitel, L. K.; Fon, E. A.; Durcan, T.

doi:10.1101/2022.02.25.482014

bioRxiv · 10.1101/2022.02.25.482014

Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing

Chen, C. X.- Q.·You, Z.·Abdian, N.·Sirois, J.·Shlaifer, I.·Tabatabaei, M.·Boivin, M.-N.·Gaborieau, L.·Karamchandani, J.·Beitel, L. K.·Fon, E. A.·Durcan, T.

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Abstract

Autosomal recessive mutations in either PRKN or PINK1 are associated with early-onset Parkinsons disease. The corresponding proteins, PRKN, an E3 ubiquitin ligase, and the mitochondrial serine/threonine-protein kinase PINK1 play a role in mitochondrial quality control. Using CRISPR/CAS9 technology we generated three human iPSC lines from the well characterized AIW002-02 control line. These isogenic iPSCs contain homozygous knockouts of PRKN (PRKN-KO, CBIGi001-A-1), PINK1 (PINK1-KO, CBIGi001-A-2) or both PINK1 and PRKN (PINK1-KO/PRKN-KO, CBIGi001-A-3). The knockout lines display normal karyotypes, express pluripotency markers and upon differentiation into relevant brain cells or midbrain organoids may be valuable tools to model Parkinsons disease.

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Chen, C. X.- Q., You, Z., Abdian, N., Sirois, J., Shlaifer, I., Tabatabaei, M., Boivin, M.-N., Gaborieau, L., Karamchandani, J., Beitel, L. K., Fon, E. A., Durcan, T.. 2022-02-25. Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing. https://doi.org/10.1101/2022.02.25.482014

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