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bioRxiv · 10.1101/2022.02.21.481375

The Zpr-3 antibody recognizes the 320-354 region of Rho and labels both rods and green cones in zebrafish

Gao, P.·Qin, Y.·Qu, Z.·Huang, Y.·Liu, X.·Li, J.·Liu, F.·Liu, M.
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Abstract

Retinal markers with high quality and specificity are important for the observation of pathologic changes of retinal cells during retinal development, degeneration, and regeneration. Zpr-3 is a mouse monoclonal antibody with uncharacterized antigen usually used to label rod photoreceptors. In this study, we provided evidence to demonstrate that the antigen gene of zpr-3 is rho, which encodes the rod opsin, and the exact epitope of zpr-3 is the 320-354 region of Rho protein. More importantly, our immunofluorescence assays indicated that zpr-3 labels both the outer segments of rods and green-cones on zebrafish retinal sections, probably due to the cross-reaction with the green-cone opsin. Our work is valuable for the scientific community to interpret the experimental data involving the zpr-3 antibody.

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Gao, P., Qin, Y., Qu, Z., Huang, Y., Liu, X., Li, J., Liu, F., Liu, M.. 2022-02-22. The Zpr-3 antibody recognizes the 320-354 region of Rho and labels both rods and green cones in zebrafish. https://doi.org/10.1101/2022.02.21.481375

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