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bioRxiv · 10.1101/2022.02.19.481155

Changes in nanomechanical properties of single neuroblastoma cells as a model for oxygen and glucose deprivation (OGD)

Zielinski, T.·Pabijan, J.·Zapotoczny, B.·Zemla, J.·Wesolowska, J.·Pera, J.·Lekka, M.
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Abstract

The biological processes underlying ischemic stroke, although complex, are better known than those related to biomechanical alterations of single cells. Mechanisms of biomechanical changes and their relations to the molecular processes are crucial for understanding the function and dysfunction of the brain. In our study, we applied atomic force microscopy (AFM) to quantify the alterations in biomechanical properties in neuroblastoma SH-SY5Y cells subjected to oxygen and glucose deprivation (OGD) and reoxygenation (RO). Obtained results reveal several characteristics. Cell viability remained at the same level, regardless of the OGD and RO conditions, but, in parallel, the metabolic activity of cells decreased with OGD duration. 24h RO did not recover the metabolic activity fully. Cells subjected to OGD appeared softer than control cells. Cell softening was strongly present in cells after 1h of OGD and, with longer OGD duration and in RO conditions, cells recovered their mechanical properties. Changes in the nanomechanical properties of cells were attributed to the remodelling of actin filaments, which was related to cofilin-based regulation and impaired metabolic activity of cells. The presented study shows the importance of nanomechanics in research on ischemic-related pathological processes such as stroke.

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Zielinski, T., Pabijan, J., Zapotoczny, B., Zemla, J., Wesolowska, J., Pera, J., Lekka, M.. 2022-02-20. Changes in nanomechanical properties of single neuroblastoma cells as a model for oxygen and glucose deprivation (OGD). https://doi.org/10.1101/2022.02.19.481155

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