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bioRxiv · 10.1101/2022.02.16.480635

FAM21 is critical for TLR2-CLEC4E-mediated dendritic cell function against Candida albicans

Abstract

FAM21 (family with sequence similarity 21) is a component of the Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) protein complex that mediates actin polymerization at endosomal membranes to facilitate sorting of cargo-containing vesicles out of endosomes. To study the function of FAM21 in vivo, we generated conditional knockout (cKO) mice in the C57BL/6 background in which FAM21 was specifically knocked out of CD11c-positive dendritic cells. Bone marrow-derived dendritic cells (BMDC) from those mice displayed enlarged early endosomes, and altered cell migration and morphology relative to wildtype (WT) cells. FAM21-cKO cells were less competent in phagocytosis and antigen processing in vitro, though antigen presentation was not affected. More importantly, we identified the TLR2/CLEC4E signaling pathway as being downregulated in FAM21-cKO BMDCs when challenged with its specific ligand Candida albicans. Moreover, FAM21-cKO mice were more susceptible to C. albicans infection than WT mice. Reconstitution of WT BMDCs in FAM21-cKO mice rescued them from lethal C. albicans infection. Thus, our study highlights the importance of FAM21 in a host immune response against a significant pathogen.

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BibTeXRIS

Kulkarni, R., Kasani, S. K., Tsai, C.-Y., Tung, S.-Y., Yeh, K.-H., Yu, C.-H. A., Chang, W.. 2022-02-17. FAM21 is critical for TLR2-CLEC4E-mediated dendritic cell function against Candida albicans. https://doi.org/10.1101/2022.02.16.480635

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