Omission responses in field potentials but not spikes in rat auditory cortex

Abstract

Non-invasive recordings of gross neural activity in humans often show responses to omitted stimuli in steady trains of identical stimuli. This has been taken as evidence for the neural coding of prediction or prediction error. However, evidence for such omission responses from invasive recordings of cellular-scale responses in animal models is scarce. Here, we sought to characterise omission responses using extracellular recordings in the auditory cortex of anaesthetised rats. We profiled omission responses across local field potentials (LFP), analogue multiunit activity (AMUA), and single/multi-unit spiking activity, using stimuli that were fixed-rate trains of acoustic noise bursts where 5% of bursts were randomly omitted. Significant omission responses were observed in LFP and AMUA signals, but not in spiking activity. These omission responses had a lower amplitude and longer latency than burst-evoked sensory responses, and omission response amplitude increased as a function of the number of preceding bursts. Contrary to theories of neural entrainment, rhythmic stimulus presentation did not increase low-frequency phase-locking of neural activity specific to the stimulus presentation rate. Together, our findings show that omission responses are observed in LFP and AMUA signals, with laminar specificity, but are not observed in spiking activity, and do not show evidence for low-frequency phase locking. This has implications for models of cortical processing that require many neurons to encode prediction error in their spike output, and may have some consistency with representation of error in dendrites electrotonically distant from the soma.