A new immunocompetent rectal cancer model to study radiation therapy

Kim, J. K.; Wu, C.; Del Latto, M.; Gao, Y.; Choi, S.-H.; Kierstead, M.; Sauve, C.-E. G.; Firat, C.; Perez, A. C.; Sillanpaa, J.; Chen, C.-T.; Lawrence, K. E.; Paty, P. B.; Barriga, F. M.; Shia, J.; Sawyers, C. L.; Lowe, S. W.; Garcia-Aguilar, J.; Romesser, P. B.; Smith, J. J.

doi:10.1101/2022.02.07.479335

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bioRxiv · 10.1101/2022.02.07.479335

A new immunocompetent rectal cancer model to study radiation therapy

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Abstract

We describe a new mouse model of rectal cancer (RC) involving rapid tumor organoid engraftment via orthotopic transplantation; the resulting RC tumors invaded inward from the mucosal surface and metastasized to distant organs. Histologically the tumors closely resemble human RC and mirror remodeling of the tumor microenvirnoment (TME) in response to radiation. This murine RC model thus recapitulates the pathogenesis of human RC, thereby fulfilling the need for a physiologically accurate model for preclinical efficacy studies.

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Kim, J. K., Wu, C., Del Latto, M., Gao, Y., Choi, S.-H., Kierstead, M., Sauve, C.-E. G., Firat, C., Perez, A. C., Sillanpaa, J., Chen, C.-T., Lawrence, K. E., Paty, P. B., Barriga, F. M., Shia, J., Sawyers, C. L., Lowe, S. W., Garcia-Aguilar, J., Romesser, P. B., Smith, J. J.. 2022-02-10. A new immunocompetent rectal cancer model to study radiation therapy. https://doi.org/10.1101/2022.02.07.479335

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