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bioRxiv · 10.1101/2022.02.04.479096

Effect of compound Tinglizi Decoction on non-classical pathway of pyroptosis in rats with chronic obstructive pulmonary disease related pulmonary hypertension

Wang, Q.·Liu, M.·Liu, Y.·Bai, Z.
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Abstract

ObjectiveTo observe the effect of compound Tinglizi Decoction on non-classical pathway of cell death in rats with pulmonary hypertension, and to explore its mechanism of action in the treatment of COPD-related pulmonary hypertension. Methods60 male SD rats were randomly divided into Western group, normal group, model group, high-dose group, medium dose group and low-dose group, with 10 rats per group. We detected the protein expression levels of caspase-11, GSDMD and IL-6 by Western blot, detected serum TNF- by ELISA, and observed the morphology of lung tissue by hematoxylin and eosin (HE) staining. ResultsIn the expression of caspase11, GSDMD, IL-6 mRNA and protein in lung tissue, the model group was significantly higher than the normal group (P < 0.01), and the high-dose group, middledose group, low-dose group and western medicine group were significantly lower than the model group (P < 0.01). The levels of serum TNF- and serum creatinine in the model group were significantly higher than those in the normal group (P < 0.01); and those in the high-dose group, middle-dose group, low-dose group and western medicine group were significantly lower than those in the model group (P < 0.05). ConclusionThe mechanism of compound Tinglizi Decoction in the intervention of COPD-related pulmonary hypertension rats is related to the inhibition of non-classical pathway of cell death.

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Wang, Q., Liu, M., Liu, Y., Bai, Z.. 2022-02-04. Effect of compound Tinglizi Decoction on non-classical pathway of pyroptosis in rats with chronic obstructive pulmonary disease related pulmonary hypertension. https://doi.org/10.1101/2022.02.04.479096

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