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bioRxiv · 10.1101/2022.02.01.478160

Profilin 1 deficiency drives mitotic defects and impairs genome stability

Abstract

Profilin 1 -encoded by PFN1- is a small actin-binding protein with a tumour suppressive role in various adenocarcinomas and pagetic osteosarcomas. However, its contribution to tumour development is not fully understood. Using fix and live cell imaging, we report that Profilin 1 inactivation results in multiple mitotic defects, manifested prominently by anaphase bridges, multipolar spindles, misaligned and lagging chromosomes, and cytokinesis failures. Accordingly, next-generation sequencing technologies highlighted that Profilin 1 knock-out cells display extensive copy-number alterations, which are associated with complex genome rearrangements and chromothripsis events in primary pagetic osteosarcomas with Profilin 1 inactivation. Mechanistically, we show that Profilin 1 is recruited to the spindle midzone at anaphase, and its deficiency reduces the supply of actin filaments to the cleavage furrow during cytokinesis. The mitotic defects are also observed in mouse embryonic fibroblasts and mesenchymal cells deriving from a newly generated knock-in mouse model harbouring a Pfn1 loss-of-function mutation. Furthermore, nuclear atypia is also detected in histological sections of mutant femurs. Thus, our results indicate that Profilin 1 has a role in regulating cell division, and its inactivation triggers mitotic defects, one of the major mechanisms through which tumour cells acquire chromosomal instability.

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BibTeXRIS

Scotto di Carlo, F., Russo, S., Muyas, F., Mangini, M., Pazzaglia, L., Biamonte, F., De Luca, A. C., Scotlandi, K., Cortes-Ciriano, I., Gianfrancesco, F.. 2022-02-01. Profilin 1 deficiency drives mitotic defects and impairs genome stability. https://doi.org/10.1101/2022.02.01.478160

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