Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

Huyke, D. A.; Ramachandran, A.; Bashkirov, V. I.; Kotseroglou, E. K.; Kotseroglou, T.; Santiago, J. G.

doi:10.1101/2022.01.31.478567

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bioRxiv · 10.1101/2022.01.31.478567

Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

Huyke, D. A.·Ramachandran, A.·Bashkirov, V. I.·Kotseroglou, E. K.·Kotseroglou, T.·Santiago, J. G.

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Abstract

Interest in CRISPR diagnostics continues to increase. CRISPR-Cas12 and -Cas13 based detection are particularly interesting as they enable highly specific detection of nucleic acids. The fundamental sensitivity limits of Cas12 and Cas13 enzymes are governed by their kinetic rates and are critical to develop amplification-free assays. However, these kinetic rates remain poorly understood and their reporting has been inconsistent. We here measure kinetic parameters for several enzymes (LbCas12a, AsCas12a, AapCas12b, LwaCas13a and LbuCas13a) and evaluate their limits of detection (LoD) for amplification-free target detection. Collectively, we here present quantitation of enzyme kinetics for 14 gRNAs and nucleic acid targets for a total of 50 sets of kinetic rate parameters and 25 LoDs. Importantly, we also validate the self-consistency our measurements by comparing trends and limiting behaviors with a Michaelis-Menten, trans-cleavage reaction kinetics model. Our measurements reveal that activated Cas12 and Cas13 enzymes exhibit typical trans-cleavage catalytic efficiencies between order 105 and 106 M-1 s-1. Moreover, for assays that use fluorescent reporter molecules (ssDNA and ssRNA) for target detection, we find most CRISPR enzymes have an amplification-free LoD in the picomolar range. We find also that successful detection of target requires cleavage (by activated CRISPR enzyme) of at least ~0.1% of the fluorescent reporter molecules. This fraction of cleaved reporters is required to differentiate signal from background, and we hypothesize that this fraction is largely independent of the detection method (i.e., endpoint vs reaction velocity). Our results provide a map of the feasible application range and highlight areas of improvement for the emerging field of CRISPR diagnostics.

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Huyke, D. A., Ramachandran, A., Bashkirov, V. I., Kotseroglou, E. K., Kotseroglou, T., Santiago, J. G.. 2022-02-01. Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics. https://doi.org/10.1101/2022.01.31.478567

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