Genomic and phylogenetic analysis of the first myovirus isolated from Oceanospirillaceae, representing a novel viral cluster prevalent in polar oceans

Zhang, W.; Liu, Y.; Xing, J.; Zheng, K.; Li, Q.; Gu, C.; Wang, Z.; Shao, H.; Guo, C.; He, H.; Wang, H.; Sung, Y. Y.; Mok, W. J.; Wong, L. L.; Liang, Y.; Wang, M.; McMinn, A.

doi:10.1101/2022.01.27.478124

bioRxiv · 10.1101/2022.01.27.478124

Genomic and phylogenetic analysis of the first myovirus isolated from Oceanospirillaceae, representing a novel viral cluster prevalent in polar oceans

Zhang, W.·Liu, Y.·Xing, J.·Zheng, K.·Li, Q.·Gu, C.·Wang, Z.·Shao, H.·Guo, C.·He, H.·Wang, H.·Sung, Y. Y.·Mok, W. J.·Wong, L. L.·Liang, Y.·Wang, M.·McMinn, A.

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Abstract

The marine bacterial family Oceanospirillaceae, which is abundant in the deep-seas and polar oceans, is closely associated with algal blooms and petroleum hydrocarbons degradation. However, only a few Oceanospirillaceae-infecting phages have so far been reported. Here we report on a novel Oceanospirillum phage, vB_OsaM_PD0307, which is the first myovirus to be found that infects Oceanospirillaceae. vB_OsaM_PD0307 with a 44,421 bp linear dsDNA genome. Phylogenetic analysis and average nucleotide sequence identities suggest that vB_OsaM_PD0307 is different from other phage isolates and represents a novel genus-level myoviral cluster with two high-quality uncultured viral genomes, designed as Oceanospimyovirus. Additionally, the biogeographical distribution of the vB_OsaM_PD0307 cluster suggests that they are widespread in the oceans and abundant in polar areas. In summary, our findings expand the current understanding of the phylogenetic diversity, genomic characteristic and function of Oceanospimyovirus phages, and highlight the role of the vB_OsaM_PD0307 phage as a major ecological agent that can infect certain key bacterial groups associated with polar algal blooms. ImportanceOceanospirillumphage vB_OsaM_PD0307 is the first myovirus found to infect Oceanospirillaceae and represents a novel viral genus, Oceanospimyovirus. This study provides insights into the genomic, phylogenetic, and ecological characteristics of myoviruses infecting Oceanospirillaceae and improves our understanding of the interactions between Oceanospirillaceae and their phages in the oceans.

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Zhang, W., Liu, Y., Xing, J., Zheng, K., Li, Q., Gu, C., Wang, Z., Shao, H., Guo, C., He, H., Wang, H., Sung, Y. Y., Mok, W. J., Wong, L. L., Liang, Y., Wang, M., McMinn, A.. 2022-01-28. Genomic and phylogenetic analysis of the first myovirus isolated from Oceanospirillaceae, representing a novel viral cluster prevalent in polar oceans. https://doi.org/10.1101/2022.01.27.478124

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