Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses

Engebrecht, J.; Li, Q.; Kaur, A.; Mallory, B.; Hariri, S.

doi:10.1101/2022.01.27.478040

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bioRxiv · 10.1101/2022.01.27.478040

Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses

Engebrecht, J.·Li, Q.·Kaur, A.·Mallory, B.·Hariri, S.

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Abstract

Biological sex affects numerous aspects of biology, yet how sex influences different biological processes has not been extensively studied at the molecular level. Caenorhabditis elegans, with both hermaphrodites (functionally females as adults) and males, is an excellent system to uncover how sex influences physiology. Here, we describe a method to isolate large quantities of C. elegans males by conditionally degrading DPY-27, a component of the dosage compensation complex essential for hermaphrodite, but not male, development. We show that germ cells from males isolated following DPY-27 degradation undergo meiosis and spermiogenesis like wild type and are competent to mate and produce viable offspring. We demonstrate the efficacy of this system by analyzing gene expression and performing affinity pull-downs from male worm extracts.

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Engebrecht, J., Li, Q., Kaur, A., Mallory, B., Hariri, S.. 2022-01-28. Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. https://doi.org/10.1101/2022.01.27.478040

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