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bioRxiv · 10.1101/2022.01.25.477707

Functional and structural deficiencies of Gemin5 variants associated with neurological disease

Abstract

Dysfunction of RNA-binding proteins are often linked to a wide range of human disease in general, and particularly with neurological conditions. Gemin5 is a member of the survival of motor neuron (SMN) complex, a ribosome-binding protein and a translation reprogramming factor. Recently, pathogenic mutations in Gemin5 protein have been reported but the functional consequences of these variants remain elusive. Here we report functional and structural deficiencies associated with compound heterozygosity variants within the Gemin5 gene found in patients with neurodevelopmental disorders. These clinical variants are located in key domains of Gemin5, the tetratricopeptide repeat (TPR)-like dimerization module and the non-canonical RNA-binding site 1 (RBS1). We show that the TPR-like variants disrupt protein dimerization while the RBS1 variant confers protein instability. All mutants are defective in the interaction with protein networks involved in translation and RNA-driven pathways. Importantly, the TPR-like variants fail to associate with native ribosomes, abolishing the cap-dependent and selective translation control of Gemin5, and establishing a functional difference with the wild type protein. Our study provides a molecular basis of disease associated with malfunction of Gemin5 protein.

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Francisco-Velilla, R., Embarc-Buh, A., del Cano-Ochoa, F., Abellan, S., Vilar, M., Alvarez, S., Fernandez-Jaen, A., Kour, S., Rajan, D., Pandey, U. B., Ramon-Maiques, S., Martinez-Salas, E.. 2022-01-25. Functional and structural deficiencies of Gemin5 variants associated with neurological disease. https://doi.org/10.1101/2022.01.25.477707

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