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bioRxiv · 10.1101/2022.01.21.477208

miniTurbo-based interactomics of two plasma membrane-localized SNARE proteins in Marchantia polymorpha

Melkonian, K.·Stolze, S. C.·Harzen, A.·Nakagami, H.
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Abstract

O_LIMarchantia polymorpha is a model liverwort and its overall low genetic redundancy is advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools in recent years. However, interactomics studies applying proximity labelling are currently limited to angiosperm species in plants. C_LIO_LIHere, we established and evaluated a miniTurbo-based interactomics method in M. polymorpha using MpSYP12A and MpSYP13B, two plasma membrane- localized SNARE proteins, as baits. C_LIO_LIWe show that our method yields a manifold of potential interactors of MpSYP12A and MpSYP13B compared to a co-immunoprecipitation approach. Our method could capture specific candidates for each SNARE. C_LIO_LIWe conclude that a miniTurbo-based method is a feasible tool for interactomics in M. polymorpha and potentially applicable to other model bryophytes. Our interactome dataset on MpSYP12A and MpSYP13B will be a useful resource to elucidate the evolution of SNARE functions. C_LI

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Melkonian, K., Stolze, S. C., Harzen, A., Nakagami, H.. 2022-01-25. miniTurbo-based interactomics of two plasma membrane-localized SNARE proteins in Marchantia polymorpha. https://doi.org/10.1101/2022.01.21.477208

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