Broad-Spectrum Antimicrobial Potential of Crassula ovata

Agbedanu, P. N.; Puga, T. B.; Schafer, J.; Buffalo, A.

doi:10.1101/2022.01.20.477177

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bioRxiv · 10.1101/2022.01.20.477177

Broad-Spectrum Antimicrobial Potential of Crassula ovata

Agbedanu, P. N.·Puga, T. B.·Schafer, J.·Buffalo, A.

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Abstract

This study investigates the antimicrobial properties of Crassula ovata (C. ovata) against both gram-positive and gram-negative bacteria. About 1-3g samples of C. Ovata leaf samples were extracted with 95 % ethanol and the extract infused into paper discs by soaking and drying. The dried discs were screened against various strains of bacteria and the antimicrobial effects of the infused agents determined by measuring zones of inhibition due to the agents infused into the discs. By the zones of inhibition, C. ovata showed antimicrobial activity against the following gram-negative bacteria: E. coli (14 mm mean zone of clearing), P. vulgaris (13 mm mean zone of clearing), E. cloacae (16 mm mean zone of clearing), and K. pneumoniae (13 mm mean zone of clearing). C. ovata showed antimicrobial activity against the following gram-positive bacteria: S. aureus (22 mm mean zone of clearing) and S. agalactiae (8 mm mean zone of clearing). C. ovata did not show antimicrobial activity against S. pyogenes. By its antimicrobial activity against gram-positive and gram-negative bacteria, C. ovata displayed broad spectrum antimicrobial activity against E. coli, S. aureus, S. agalactiae, P. vulgaris, E. cloacae, and K. pneumoniae. C. ovata may have the potential to serve as a broad-spectrum antimicrobial in the future. Further testing should be done to investigate the toxicities and side effects of C. ovata. Further testing must also be done with C. ovata against drug-resistant strains of bacteria.

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Agbedanu, P. N., Puga, T. B., Schafer, J., Buffalo, A.. 2022-01-25. Broad-Spectrum Antimicrobial Potential of Crassula ovata. https://doi.org/10.1101/2022.01.20.477177

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