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bioRxiv · 10.1101/2022.01.19.476895

Probing the proton release by Photosystem II in the S1 to S2 high-spin transition

Abstract

The stoichiometry and kinetics of the proton release were investigated during each transition of the S-state cycle in Photosystem II (PSII) from Thermosynechococcus elongatus containing either a Mn4CaO5 (PSII/Ca) or a Mn4SrO5 (PSII/Sr) cluster. The measurements were done at pH 6.0 and pH 7.0 knowing that, in PSII/Ca at pH 6.0 and pH 7.0 and in PSII/Sr at pH 6.0, the flash-induced S2-state is in a low-spin configuration (S2LS) whereas in PSII/Sr at pH 7.0, the S2-state is in a high-spin configuration (S2HS) in half of the centers. Two measurements were done; the time-resolved flash dependent i) absorption of either bromocresol purple at pH 6.0 or neutral red at pH 7.0 and ii) electrochromism in the Soret band of PD1 at 440 nm. The fittings of the oscillations with a period of four indicate that one proton is released in the S1 to S2HS transition in PSII/Sr at pH 7.0. It has previously been suggested that the proton released in the S2LS to S3 transition would be released in a S2LSTyrZ[bullet] [->] S2HSTyrZ[bullet] transition before the electron transfer from the cluster to TyrZ[bullet] occurs. The release of a proton in the S1TyrZ[bullet] [->]S2HSTyrZ transition would logically imply that this proton release is missing in the S2HSTyrZ[bullet] to S3TyrZ transition. Instead, the proton release in the S1 to S2HS transition in PSII/Sr at pH 7.0 was mainly done at the expense of the proton release in the S3 to S0 and S0 to S1 transitions. However, at pH 7.0, the electrochromism of PD1 seems larger in PSII/Sr when compared to PSII/Ca in the S3 state. This points to the complex link between proton movements in and immediately around the Mn4 cluster and the mechanism leading to the release of protons into the bulk.

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BibTeXRIS

Boussac, A., sugiura, m., sellés, j.. 2022-01-20. Probing the proton release by Photosystem II in the S1 to S2 high-spin transition. https://doi.org/10.1101/2022.01.19.476895

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