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bioRxiv · 10.1101/2022.01.15.476435

The secreted metabolome of HeLa cells under effect of Crotamine, a cell-penetrating peptide from a rattlesnake using NMR-based metabolomics analyses

Abstract

Sequestering and reprogramming of cellular metabolism represents one of the principal hallmarks several cells. Antimicrobial peptides have been shown to exhibit selective anticancer activities. In this study, the secreted metabolome of HeLa cells under action of the antimicrobial peptide Crotamine was evaluated. Although, Crotamine has been shown to be selective for highly proliferating cells and is able to extend the in vivo lifespan. The present study using a cell line of cervical cancer, HeLa cells provide insights into how Crotamine acts in cell metabolism. NMR spectroscopy was used to identify and quantify relative metabolite levels, which are associated with Crotamine uptake. Statistical analysis reveals that Crotamine dramatically affects metabolites related to glycolysis, metabolism and biosynthesis of amino acids and pyruvate metabolism. The developed machine learning model is found to be robust by ROC curve analysis, suggesting that the metabolic state of HeLa cells treated with Crotamine is different from the control samples. To account for metabolite levels, it is suggested that Crotamine would have to act on glycolysis, which, in turn, affects several other metabolic pathways, such as, glutathione metabolism, TCA cycle and pyruvate metabolism. The observed metabolic changes shed light into the mode of Crotamine function.

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BibTeXRIS

Coronado, M. A., de Moraes, F. R., Stuqui, B., Calmon, M. F., Eberle, R. J., Rahal, P., Arni, R. K.. 2022-01-18. The secreted metabolome of HeLa cells under effect of Crotamine, a cell-penetrating peptide from a rattlesnake using NMR-based metabolomics analyses. https://doi.org/10.1101/2022.01.15.476435

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