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bioRxiv · 10.1101/2022.01.14.476362

Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to mouse lumbar intervertebral disc

Walk, R. E.·Moon, H. J.·Tang, S. Y.·Gupta, M. C.
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Abstract

Study DesignPreclinical animal study ObjectiveEvaluation of the degenerative progression resulting from either a partial- or full- width injury to the mouse lumbar intervertebral disc (IVD) using contrast-enhanced micro-computed tomography and histological analyses. We utilized a lateral-retroperitoneal surgical approach to access the lumbar IVD, and the injuries to the IVD were induced by either incising one side of the annulus fibrosus or puncturing both sides of the annulus fibrosus. The full-width injury caused dramatic reduction in nucleus pulposus hydration and significant degeneration. A partial-width injury produces localized deterioration around the annulus fibrosus that resulted in local tissue remodeling without gross degeneration to the IVD. MethodsFemale C57BL/6J mice of 3-4 months age were used in this study. They were divided into three groups to undergo partial-width, full-width, or sham injuries. The L5/L6 and L6/S1 lumbar IVDs were surgically exposed using a lateral-retroperitoneal approach. The L6/S1 IVDs were injured using either a surgical scalpel (partial-width) or a 33G needle (full-width), with the L5/L6 serving as an internal control. These animals were allowed to recover and then sacrificed at 2-, 4-, or 8-weeks post-surgery. The IVDs were assessed for degeneration using contrast-enhanced microCT (CE{micro}CT) and histological analysis. ResultsThe high-resolution 3D evaluation of the IVD confirmed that the respective injuries localized within one side of the annulus fibrosus or spanned the full width of the IVD. The full-width injury caused deteriorations in the nucleus pulposus after 2 weeks and progressed to significant degeneration at 8 weeks, while the partial width injury caused localized disruptions that remained limited to the annulus fibrosus. ConclusionThe use of CE{micro}CT revealed distinct IVD degeneration profiles resulting from partial- and full- width injuries. The partial width injury may serve as an alternative for IVD degeneration resulting from localized annulus fibrosus injuries in humans.

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Walk, R. E., Moon, H. J., Tang, S. Y., Gupta, M. C.. 2022-01-16. Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to mouse lumbar intervertebral disc. https://doi.org/10.1101/2022.01.14.476362

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