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bioRxiv · 10.1101/2022.01.05.475094

New insights on the role of HCN in root hair elongation through single cell proteomics

Garcia, I.·Arenas-Alfonseca, L.·Romero, L. C.·Yamada, M.
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Abstract

Root hairs are specialized structures involved in water and nutrient uptake by plants. They elongate from epidermal cells following a complex developmental program. {beta}-cyanoalanine synthase (CAS), which is mainly involved in hydrogen cyanide (HCN) detoxification in Arabidopsis thaliana, plays a role in root hair elongation, as evidenced by the fact that cas-c1 mutants show a severe defect in root hair shape. In addition to root hairs, CAS C1 is expressed in the quiescent center and meristem. However, the cas-c1 mutation has no visible effect on either tissue, in both control and nutrient-deprivation conditions. To identify its role in root hair formation, we conducted single cell proteomics analysis by isolating root hair cells using Fluorescence-Activated Cell Sorting (FACS) from wild type and cas-c1 mutants. We also analyzed the presence of S-cyanylation, a protein post-translational modification (PTM) mediated by HCN and affecting cysteine residues and protein activity, in proteins of wild type and cas-c1 mutants. We found that several proteins involved in root hair development, related to the receptor kinase FERONIA signaling and to DNA methylation, are modified by this new post-translational modification. One sentence summaryArabidopsis root hair proteomics unveals that several proteins involved in root hair development are susceptible of modification by S-cyanylation.

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Garcia, I., Arenas-Alfonseca, L., Romero, L. C., Yamada, M.. 2022-01-07. New insights on the role of HCN in root hair elongation through single cell proteomics. https://doi.org/10.1101/2022.01.05.475094

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