Cryo-EM structure of the human CST·Polα/Primase complex in a recruitment state

Cai, S. W.; Zinder, J. C.; Svetlov, V.; Bush, M.; Nudler, E.; Walz, T.; de Lange, T.

doi:10.1101/2021.12.17.473235

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bioRxiv · 10.1101/2021.12.17.473235

Cryo-EM structure of the human CST·Polα/Primase complex in a recruitment state

Cai, S. W.·Zinder, J. C.·Svetlov, V.·Bush, M.·Nudler, E.·Walz, T.·de Lange, T.

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Abstract

The CST*Pol/Primase complex is essential for telomere overhang maintenance and additionally functions to counteract resection at double-strand breaks. We report a 4.6-[A] resolution cryo-EM structure of CST*Pol/Primase, captured prior to catalysis in a recruitment state, which provides insights into the architecture and stoichiometry of the fill-in machinery. Our model informs on human disease mutations that cause Coats plus syndrome.

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Cai, S. W., Zinder, J. C., Svetlov, V., Bush, M., Nudler, E., Walz, T., de Lange, T.. 2021-12-18. Cryo-EM structure of the human CST·Polα/Primase complex in a recruitment state. https://doi.org/10.1101/2021.12.17.473235

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