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bioRxiv · 10.1101/2021.12.17.473010

Well-Lit: A programmable and customizable assistant for manual multi-well plate pipetting

Gomez-Sjöberg, R.·Cabrera, J. P.·Cote, A.
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Abstract

A very large number of biology and biochemistry laboratory protocols require transferring liquid aliquots from individual containers into individual wells of a multi-well plate, from plates to individual containers, or from one plate to another. Doing this by hand without errors, such as skipping wells, placing two samples in the same well, or swapping sample locations, especially when using plates with 96 wells or more, is difficult and requires enormous operator focus and/or a tedious manual error checking system. We present here a device built to facilitate error-free pipetting of samples from individual barcoded tubes to a multi-well plate or between multi-well plates (both 96 and 384 wells are supported). The device is programmable, modular and easily customizable to accommodate plates with different form-factors, and different protocols. The main components are only a 12.3" touch screen, a small form-factor PC, and a barcode scanner, combined with custom-made parts can be easily fabricated with a laser cutter and a hobby-grade 3D printer. The total cost is between approximately US$550 and US$600, depending on the configuration. Specifications table O_TBL View this table: org.highwire.dtl.DTLVardef@8554f4org.highwire.dtl.DTLVardef@18c8878org.highwire.dtl.DTLVardef@153b050org.highwire.dtl.DTLVardef@15c7de6org.highwire.dtl.DTLVardef@14daeb7_HPS_FORMAT_FIGEXP M_TBL C_TBL

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Gomez-Sjöberg, R., Cabrera, J. P., Cote, A.. 2021-12-20. Well-Lit: A programmable and customizable assistant for manual multi-well plate pipetting. https://doi.org/10.1101/2021.12.17.473010

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