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bioRxiv · 10.1101/2021.12.14.472592

Control of G2 phase duration by CDC25B modulates the switch from direct to indirect neurogenesis in the neocortex

Roussat, M.·Jungas, T.·Audouard, C.·Medevielle, F.·Davy, A.·Pituello, F.·Bel-Vialar, S.
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Abstract

During development, cortical neurons are produced in a temporally regulated sequence from apical progenitors, directly, or indirectly through the production of intermediate basal progenitors. The balance between these major progenitors types is determinant for the production of the proper number and types of neurons and it is thus important to decipher the cellular and molecular cues controlling this equilibrium. Here we address the role of a cell cycle regulator, the CDC25B phosphatase, in this process. We show that deleting CDC25B in apical progenitors leads to a transient increase of the production of TBR1+ neurons at the expense of TBR2+ basal progenitors in mouse neocortex. This phenotype is associated with lengthening of the G2 phase of the cell cycle, the total cell cycle length being unaffected. Using in utero electroporation and cortical slice cultures, we demonstrate that the defect in TBR2+ basal progenitor production requires interaction with CDK1 and is due to the G2 phase lengthening in CDC25B mutants. Altogether, this study identifies a new role for CDC25B and the length of the G2 phase in direct versus indirect neurogenesis at early stages of the cortical development.

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Roussat, M., Jungas, T., Audouard, C., Medevielle, F., Davy, A., Pituello, F., Bel-Vialar, S.. 2021-12-15. Control of G2 phase duration by CDC25B modulates the switch from direct to indirect neurogenesis in the neocortex. https://doi.org/10.1101/2021.12.14.472592

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