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bioRxiv · 10.1101/2021.12.13.472375

An mRNA silencing mechanism reliant on the cooperation between REGE-1/Regnase-1 and RLE-1/Roquin-1

Sobanska, D.·Komur, A. A.·Chabowska-Kita, A.·Gumna, J.·Kumari, P.·Pachulska-Wieczorek, K.·Ciosk, R.
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Abstract

Regnase-1 is an evolutionarily conserved endoribonuclease, degrading diverse mRNAs important, among others, for immune homeostasis, development, and cancer. There are two competing models of Regnase-1 mediated mRNA silencing. One model postulates that Regnase-1 works together with another RNA-binding protein, Roquin-1. The other model proposes that the two proteins function separately. Studying the C. elegans Regnase-1 ortholog, REGE-1, we have uncovered a functional relationship between REGE-1 and the nematode counterpart of Roquin-1, RLE-1. While REGE-1 and RLE-1 associate with mRNA independently of each other, both proteins are essential for mRNA silencing. Intriguingly, the functional interdependence between REGE-1 and RLE-1 is reminiscent of the proposed cooperation between mammalian Regnase-1 and Roquin-1, which may underlie a prototypic silencing mechanism involving both proteins.

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Sobanska, D., Komur, A. A., Chabowska-Kita, A., Gumna, J., Kumari, P., Pachulska-Wieczorek, K., Ciosk, R.. 2021-12-13. An mRNA silencing mechanism reliant on the cooperation between REGE-1/Regnase-1 and RLE-1/Roquin-1. https://doi.org/10.1101/2021.12.13.472375

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